Department of Molecular Chemistry and Biochemistry
Doshisha University, Kyoto, Japan



March 2001:   Bachelor of Engineering, Doshisha University


March 2003:   Master of Engineering, Nara Institute Science &


                        Supervisor: Prof. Yoshiaki Kobuke

March 2006:   Doctor of Engineering, Doshisha University

                        Supervisor: Prof. Koji Kano



April 2006

– March 2008:    Postdoctoral Fellow at Osaka University

                           Collaborator: Prof. Takashi Hayashi

April 2008

– March 2014:    Assistant Professor at Doshisha University

                           Collaborator: Prof. Koji Kano

Sept. 2009

– August 2010:    Guest Researcher at The Scripps Research


                             Collaborator: Prof. M. G. Finn

April 2014

– Present:             Associate Professor at Doshisha University


RESEARCH  FIELD: Supramolecular Chemistry, Organic Chemistry, Bioinorganic Chemistry, Biological Chemistry



  1. (1)R. Nishiyabu, Y. Takahashi, T. Yabuki, S. Gommori, Y. Yamamoto, H. Kitagishi, Y. Kubo
    “Boronate Sol-Gel Method for One-Step Fabrication of Polyvinyl Alcohol Hydrogel Coating by Simple Cast- and Dip-Coating Techniques”
    ASC Advances, published on web (2020).
    (DOI: 10.1039/C9RA08208E)

  1. (2)H. Kitagishi
    “In Vivo Reactions Triggered by Selective Removal of Endogenous Carbon Monoxide in Blood Using an Artificial Hemoprotein Model Complex”
    Artificial Blood, 27, 44–52 (2019) (written in Japanese).

  1. (3)M. Vonesch, J. A. Wytko, H. Kitagishi, K. Kano, J. Weiss
    “Modeling Haemoproteins: Porphyrins and Cyclodextrins as Sources of Inspiration”
    Chemical Communications, 27, 44–52 (2019).
    (DOI: 10.1039/C9CC07545C)

  1. (4)H. Kitagishi, M. Saito, Q. Mao, A. Kiriyama, S. Negi, K. Kano
    “Supramolecular Complexation in Biological Media. NMR Study on Inclusion of an Anionic Tetraarylporphyrin into a Heptakis(2,3,6-tri-
    O-methyl)-β-cyclodextrin Cavity in Serum, Blood, and Urine”
    Chemistry–An Asian Journal, 14, 3320–3328 (2019).

  1. (5)H. Kitagishi, K. Ohara, D. Shimoji, M. Vonesch, J. Weiss, J. A. Wytko
    “Hydrogen Bond Directed Molecular Recognition in Water in a Strapped-Porphyrin-Cyclodextrin Assembly”
    Journal of Porphyrins and Phthalocyanines, 23, 569–574 (2019).

  1. (6)R. Suzuki, Y. Inoue, I. Murata, H. Nomura, Y. Issiki, M. Hashimoto, Y. Kudo, H. Kitagishi, S. Kondo, I. Kanamoto
    “Preparation, Characterization, and Study of the Antimicrobial Activity of a Hinokitiol-copper(II)/γ-cyclodextrin Ternary Complex”
    Journal of Molecular Structure, 1194, 9–27 (2019).

  1. (7)M. Kodera, Y. Kadoya, K. Aso, K. Fukui, A. Nomura, Y. Hitomi, H. Kitagishi
    “Acceleration of Hydrolytic DNA Cleavage by Dicopper(II) Complexes with
    p-Cresol-Derived Dinulearing Ligands at Slightly Acidic pH and Mechanistic Insights”
    Bulletin of the Chemical Society of Japan, 92, 739–747 (2019).

  1. (8)A. Ollivier, R. Foresti, Z. El Ali, T. Martens, H. Kitagishi, R. Motterlini, M. Rivard
    “Design and Biological Evaluation of Manganese- and Ruthenium-based Hybrid CO-RMs (HYCOs)”
    ChemMedChem, 14, 1684–1691 (2019).

  1. (9)K. Oohora, R. Kajihara, M. Jiromaru, H. Kitagishi, T. Hayashi
    “Arginine Residues Provide a Multivalent Effect for Cellular Uptake of a Hemoprotein Assembly”
    Chemistry Letters, 48, 295–298 (2019).

  1. (10)Q. Mao, H. Kitagishi
    “Optimized Synthesis of a Per-O-methylated
    β-Cyclodextrin Dimer Linked at the Secondary Face by a Pyridine Ligand”
    Journal of Inclusion Phenomena and Macrocyclic Chemistry, 93, 67–76 (2019).

  1. (11)L. Braud, M. Pini, L. Muchova, S. Manin, H. Kitagishi, D. Sawaki, G. Czibik, J. Ternacle, G. Derumeaux, R. Foresti, R. Motterlini
    “Carbon Monoxide-induced Metabolic Switch in Adipocytes Improves Insulin Resistance in Obese Mice”
    JCI Insight, 3, e123485 (2018).

  1. (12)A. T. Kawaguchi, A. Salybekov, M. Yamano, H. Kitagishi, K. Sekine
    “PEGylated Carboxyhemoglobin Bovine (SUNGUINATE) Ameliorates Myocardial Infatction in a Rat Model”
    Artificial Organs, 42, 1174–1184 (2018).

  1. (13)K. Ueno, J.Morstein, K. Ofusa, S. Naganos, E. Suzuki-Sawano, A. Minegishi, H. Kitagishi, C. J. Chang, J. Horiuchi, M. Saitoe
    “Carbon Monoxide, a Retrograde Messenger Generated in Post-Synaptic Mushroom Body Neurons Evokes Local Dopamin release”
    bioRxiv Neuroscience, 42, 1–46 (2018).

  1. (14)S. Minegishi, I. Sagami, S. Negi, K. Kano, H. Kitagishi
    “Circadian Clock Disruption by Selective Removal of Endogenous Carbon Monoxide”
    Scientific Reports, 8, 11996 (2018).
    (DOI: 10.1038/s41598-018-30425-6)

  1. (15)H. Kitagishi, D. Shimoji, T. Ohta, R. Kamiya, Y. Kudo, A. Onoda, T. Hayashi, J. Weiss, J. A. Wytko, K. Kano
    “A Water-Soluble Supramolecular Complex that Mimics the Heme/Copper Hetero-Binuclear Site of Cytochrome
    c Oxidase”
    Chemical Science, 9, 1989–1995 (2018).
    (DOI: 10.1039/C7SC04732K)

  2. (16)S. Minegishi, A. Yumura, H. Miyoshi, S. Negi, S. Taketani, R. Motterlini, R. Foresti, K. Kano, H. Kitagishi
    “Detection and Removal of Endogenous Carbon Monoxide by Selective and Cell-permeable Hemoprotein-model Complexes”
    Journal of the American Chemical Society, 139, 5984–5991 (2017).
    (DOI: 10.1021/jacs.7b02229)

  1. (17)H. Kitagishi, S. Minegishi
    “Iron(II)porphyrin–Cyclodextrin Supramolecular Complex as a Carbon Monoxide-Depleting Agent in Living Organisms
    Chemical and Pharmaceutical Bulletin, 65, 336–340 (2017).

  1. (18)H. Kitagishi, Y. Sugaya, K. Komazawa, M. Tamaki, K. Kano
    “Participation of Hydroxyl Radical in the Formation of Verdoheme-type Compound in the Reaction of a Ferric Porphyrin with Hydrogen Peroxide”
    Chemistry Letters, 46, 509–512 (2017).

  1. (19)H. Kitagishi, Q. Mao, N. Kitamura, T. Kita
    “HemoCD as a Totally Synthetic Artificial Oxygen Carrier: Improvements in the Synthesis and
    O2/CO Discrimination”
    Artificial Organs, 41, 372–380 (2017).

  1. (20)R. Nishiyabu, S. Iizuka, S. Minegishi, H. Kitagishi, Y. Kubo
    “Surface Modification of a Polyvinyl Alcohol Sponge with Functionalized Boronic Acids to Develop Porous Materials for Multicolor Emission, Chemical Sensing and 3D Cell Culture”
    Chemical Communications, 53, 3563–3566 (2017).

  1. (21)H. Kitagishi, S. Kurosawa, K. Kano
    “Intramolecular Oxidative
    O-Demethylation of an Oxoferryl Porphyrin Complexed with a Per-O-methylated β-Cyclodextrin Dimer”
    Chemistry –An Asian Jouranl, 11, 3213–3219 (2016).
    (DOI: 10.1002/asia.201601097)

  1. (22)W. Takabe, Y. Urano, D. H. Vo, K. Shibuya,  M. Tanno, H. Kitagishi, T. Fujimoto, N. Noguchi
    “Esterification of 24S-OHC Induces Formation of Atypical Lipid Droplet-like Structures, Leading to Neuronal Cell Death.
    Journal of Lipid Research, 57, 2005–2014 (2016).
    (DOI: 10.1194/jlr.M068775)

  1. (23)K. Shibuya,  T. Watanabe, Y. Urano, W. Takabe, N. Noguchi, H. Kitagishi
    “Synthesis of 24(
    S)-Hydroxycholesterol Esters Responsible for the Induction of Neuronal Cell Death”
    Bioorganic & Medicinal Chemisty, 24, 2559–2566 (2016).
    (DOI: 10.1016/j.bmc.2016.04.024)

  1. (24)H. Kitagishi, S. Minegishi, A. Yumura, S. Negi, S. Taketani, Y. Amagase, Y. Mizukawa, T. Urushidani, Y. Sugiura, K. Kano
    “Feedback Response to Selective Depletion of Endogenous Carbon Monozide in the Blood”
    Journal of the American Chemical Society, 138, 5417–5425 (2016).
    (DOI: 10.1021/jacs.6b02211)

  1. (25) K. Imabeppu, H. Kuwano, E. Yutani, H. Kitaghishi, K. Kano
    “Photoinduced Homolysis of Alkyl-Cobalt(III) Bonds in a Cyclodextrin Cage”
    European Journal of Inorganic Chemistry, 1784–1789 (2016).
    (DOI: 10.1002/ejic.201600208)

  1. (26) H. Kitagishi, H. Kawasaki, K. Kano
    “Bioconjugation of Serum Albumin to a Maleimide-appended Porphyrin/Cyclodextrin Supramolecular Complex as an Artificial Oxygen Carrier in the Bloodstream”
    Chemistry –An Asian Journal, 10, 1768–1775 (2015).
    (DOI: 10.1002/asia.201500451)

  1. (27) K. Watanabe, T. Suzuki, H. Kitagishi, K. Kano
    “Reaction Between a Haemoglobin Model Compound and Hydrosulphide in Aqueous Solution”
    Chemical Communications, 51, 4059–4061 (2015).
    (DOI: 10.1039/C5CC00057B)

  1. (28) H. Kitagishi, F. Chai, S. Negi, Y. Sugiura, K. Kano
    “Supramolecular Intracellular Delivery of an Anionic Porphyrin by Octaarginine-conjugated Per-
    Chemical Communications, 51, 2421–2424 (2015).
    (DOI: 10.1039/C4CC09042J)

  1. (29) T. Ueda, H. Kitagishi, K. Kano
    “Intramolecular Direct Oxygen Transfer from Oxoferryl porphyrin to a Sulfide Bond”
    Inorganic Chemistry, 53, 543–551 (2014).
    (DOI: 10.1021/ic4026393)

  1. (30) T. Yamagiwa, A. T. Kawaguchi, T. Saito, S. Inoue, S. Morita, K. Watanabe, H. Kitagishi, K. Kano, S. Inokuchi
    “Supramolecular Ferric Porphyrins and a Cyclodextrin Dimer as Antidotes for Cyanide Poisoning”
    Human and Experimental Toxicology, 33, 360–368 (2014).
    (DOI: 10.1177/0960327113499041)

  1. (31) H. Kitagishi, K. Minami, K. Kano
    “Intracellular Delivery of Anionic
    meso-Tetraphenylporphyrin–Per-O-methylated β-Cyclodextrin Supramolecular Complex by an Oligoarginine Carrier Peptide”
    Chemistry Letters, 43, 1095–1097 (2014).
    (DOI: 10.1246/cl.140298)

  1. (32) H. Kitagishi, S. Hatada, T. Itakura, Y. Maki, Y. Maeda, K. Kano
    “Cellular Uptake of Octaarginine-conjugated Tetraarylporphyin Included by Per-
    O-methylated β-Cyclodextrin”
    Organic & Biomolecular Chemistry, 11, 3203–3211 (2013).
    (DOI: 10.1039/c3ob27248f)

  1. (33) T. Ueda, S. Kumeda, H. Kitagishi, K. Kano
    “Intramolecular Oxidative
    O-Demethylation in a Per-O-methylated β-Cyclodextrin–Iron Porphyrin Inclusion Complex in Aqueous Solution”
    Chemistry Letters, 42, 1366–1368 (2013).
    (DOI: 10.1246/cl.130678)

  1. (34) K. Karasugi, H. Kitagishi, K. Kano
    “Elongation of Circulation Time of a Diatomic Molecule Receptor in Bloodstream by Attachment to Small Gold Nanoparticles”
    Chemistry Letters, 42, 115–117 (2013).
    (DOI: 10.1246/cl.2013.115)

  1. (35) K. Watanabe, H. Kitagishi, K. Kano
    “Supramolecular Iron Porphyrin/Cyclodextrin Dimer Complex that Mimics the Functions of Hemoglobin and Methemoglobin”
    Angewandte Chemie International Edition, 52, 6894–6897 (2013).
    (DOI: 10.1002/anie.201302470)

  1. (36) Y. Kakikura, A. Onoda, E. Kubo, H. Kitagishi, T. Uematsu, S. Kuwabata, T. Hayashi
    “Supramolecular Linear Assemblies of Cytochrome
    b562 Immobilized on a Gold Electrode”
    Journal of Inorganic and Organometallic Polymers and Materials, 23, 172–179 (2013).
    (DOI: 10.1007/s10904-012-9737-1)

  1. (37) T. Ueda, H. Kitagishi, K. Kano
    “PEGylation of an Artificial O
    2 and CO Receptor: Synthesis, Characterisation and Pharmacokinetic Study”
    Organic & Biomolecular Chemistry, 10, 4337–4347 (2012).
    (DOI: 10.1039/c2ob07044h)

  1. (38) K. Kano, S. Chimoto, M. Tamaki, Y. Itoh, H. Kitagishi
    “Supramolecular Dioxygen Receptors Composed of an Anionic Water-soluble Porphinatoiron(II) and Cyclodextrin Dimers”
    Dalton Transactions, 41, 453–461 (2012).
    (DOI: 10.1039/c1dt11596k)

  1. (39) H. Kitagishi, K. Kashiwa, K. Kano
    “Functionalization of a Protein Surface with Per-
    O-Methylated β-Cyclodextrin”
    Biopolymers, 97, 11–20 (2012).
    (DOI: 10.1002/bip.21695)

  1. (40) J.-K. Rhee, M. Baksh, C. Nycholat, J. C. Paulson, H. Kitagishi, M. G. Finn
    “Glycan-Targeted Virus-like Nanoparticles for Photodynamic Therapy”
    Biomacromolecules, 13, 2333–2338 (2012).
    (DOI: 10.1021/bm300578p)

  1. (41) K. Karasugi, H. Kitagishi, K. Kano
    “Modification of a Dioxygen Carrier, HemoCD, with PEGylated Dendrons for Extension of Circulation Time in the Bloodstream”
    Bioconjugate Chemistry, 23, 2365–2376 (2012).

  1. (42) Y. Sano, A. Onoda, R. Sakurai, H. Kitagishi, T. Hayashi
    “Preparation and Reactivity of a Tetranuclear Fe(II) Core in the Metallothionein α-domain”
    Journal of Inorganic Biochemistry, 105, 702–708 (2011).
    (DOI: 10.1016/j.jinorgbio.2011.01.011)

  1. (43) K. Kano, M. Kondo, H. Inoue, H. Kitagishi, B. Colasson, O. Reinaud
    “Calorimetric Study on Coordination of Tridentate Imidazolyl Calix[6]arene Ligand to Zinc Ion in Organic Solvents”
    Inorganic Chemistry, 50, 6353–6360 (2011).
    (DOI: 10.1021/ic200809k)

  1. (44) K. Oohora, A. Onoda, H. Kitagishi, H. Yamaguchi, A. Harada, T. Hayashi
    “A Chemically-Controlled Supramolecular Protein Polymer Formed by a Myoglobin-Based Self-Assembly Systems”
    Chemical Science, 2, 1033–1038 (2011).
    (DOI: 10.1039/c1sc00084e)

  1. (45) K. Kano, T. Ochi, S. Okunaka, Y. Ota, K. Karasugi, T. Ueda, H. Kitagishi
    “Preparation and Function of Poly(acrylic acid)s Modified by Supramolecular Complex Composed of Porphinatoiron and a Cyclodextrin Dimer That Bind Diatomic Molecules (O
    2 and CO) in Aqueous Solution”
    Chemistry –An Asian Journal, 6, 2946–2955 (2011).
    (DOI: 10.1002/asia.201100354)

  1. (46) K. Karasugi, H. Kitagishi, K. Kano
    “Gold Nanoparticles Carrying Diatomic Molecules (O
    2 and CO) in Aqueous Solution”
    Chemistry –An Asian Journal, 6, 825–833 (2011).
    (DOI: 10.1002/asia.201000756)

  1. (47) J.-K. Rhee, M. Hovlid, J. D. Fiedler, S. D. Brown, F. Manzenrieder, H. Kitagishi, C. Nycholat, J. C. Paulson, M. G. Finn
    “Colorful Virus-like Particles: Fluorescent Protein Packaging by the Q
    β Capsid”
    Biomacromolecules, 12, 3977–3981 (2011).
    (DOI: 10.1021/bm200983k)

  1. (48) K. Watanabe, H. Kitagishi, K. Kano
    “Supramolecular Ferric Porphyrins as Cyanide Receptors in Aqueous Solution”
    ACS Medicinal Chemistry Letters, 2, 943–947 (2011).
    (DOI: 10.1021/ml200231x)

  1. (49) S. Hirota, N. Tanaka, I. Micetic, P. Di Muro, S. Nagao, H. Kitagishi, K. Kano, R. S. Magliozzo, J. Peisach, M. Beltramini, L. Bubacco
    “Structural basis of the lactate-dependent allosteric regulation of oxygen binding in arthropod hemocyanin”
    The Journal of Biological Chemistry, 285, 19338–19345 (2010).
    (DOI: 10.1074/jbc.M109.076067)

  1. (50) H. Kitagishi, M. Tamaki, T. Ueda, S. Hirota, T. Ohta, Y. Naruta, K. Kano
    “Oxoferryl Porphyrin/Hydrogen Peroxide System Whose Behavior is Equivalent to Hydroperoxoferric Porphyrin”
    Journal of the American Chemical Society, 132, 16730–16732 (2010).
    (DOI: 10.1021/ja106798a)

  1. (51) H. Kitagishi, S. Negi, A. Kiriyama, A. Honbo, Y. Sugiura, A. T. Kawaguchi, K. Kano
    “A Diatomic Molecule Receptor That Removes CO in a Living Organism”
    Angewandte Chemie International Edition, 49, 1312–1315 (2010).
    (DOI: 10.1002/anie.200906149)

  1. (52) H. Kitagishi, K. Kano
    “Completely Synthetic Compounds That Bind Diatomic Molecules (O2, CO, NO) in Aqueous Solution”
    Jinko Ketsueki, 17, 160–168 (2010) (written in Japanese).

  1. (53) H. Kitagishi, K. Oohora, T. Hayashi
    “Thermodynamically Controlled Supramolecular Polymerization of Cytochrome
    Biopolymers, 91, 194–200 (2009).
    (DOI: 10.1002/bip.21114)

  1. (54) K. Kano, H. Kitagishi
    “HemoCD as an Artificial Oxygen Carrier: Oxygen Binding and Autoxidation”
    Artificial Organs, 33, 177–182 (2009).
    (DOI: 10.1111/j.1525-1594.2008.00704.x)

  1. (55) H. Kitagishi, Y. Kakikura, H. Yamaguchi, K. Oohora, A. Harada, T. Hayashi
    “Self-Assembly of One- and Two-Dimensional Hemoprotein Systems by Polymerization through Heme–Heme Pocket Interactions”
    Angewandte Chemie International Edition, 48, 1271–1274 (2009).
    (DOI: 10.1002/anie.200804006)

  1. (56) K. Kano, Y. Itoh, H. Kitagishi, T. Hayashi, S. Hirota
    “A Supramolecular Receptor of Diatomic Molecule (O2, CO, NO) in Aqueous Solution”
    Journal of the American Chemical Society, 130, 8006–8015 (2008).
    (DOI: 10.1021/ja8009583)

  1. (57) H. Kitagishi, K. Oohora, H. Yamaguchi, H. Sato, T. Matsuo, A. Harada, T. Hayashi
    “Supramolecular Hemoprotein Linear Assembly by Successive Interprotein Heme–Heme Pocket Interactions”
    Journal of the American Chemical Society, 129, 10326–10327 (2007).
    (DOI: 10.1021/ja073295q)

  1. (58) K. Kano, H. Kitagishi, S. Tanaka
    “Characterization of an Inclusion Complex of 5,10,15,20-Tetrakis(4-sulfonatophenyl)- porphinato Iron and an
    O-Methylated β-Cyclodextrin Dimer Having a Pyridine Linker and Its Related Complexes in Aqueous Solution”
    Journal of Inclusion Phenomena and Macrocyclic Chemistry, 56, 69–74 (2006).
    (DOI: 10.1007/s10847-006-9063-8)

  1. (59) K. Kano, H. Kitagishi, C. Dagallier, M. Kodera, T. Matsuo, T. Hayashi, Y. Hisaeda, S. Hirota
    “Iron porphyrin–cyclodextrin supramolecular complex as a functional model of myoglobin in aqueous solution”
    Inorganic Chemistry 45, 4448–4460 (2006).
    (DOI: 10.1021/ic060137b)

  1. (60) K. Kano, H. Kitagishi, Y. Sone, N. Nakazawa, M. Kodera
    “Redox Behavior of a Manganese Porphyrin Complexed with Per-
    O-methylated β-Cyclodextrin in Aqueous Solution”
    European Journal of Inorganic Chemistry, 2006, 4043–4053 (2006).
    (DOI: 10.1002/ejic.200600336)

  1. (61) K. Kano, H. Kitagishi, T. Mabuchi, M. Kodera, S, Hirota
    “A Myoglobin Functional Model Composed of a Ferrous Porphyrin and a Cyclodextrin Dimer with an Imidazole Linker”
    Chemistry –An Asian Journal, 1, 358–366 (2006).
    (DOI: 10.1002/asia.200600070)

  1. (62) K. Kano, H. Kitagishi, M. Kodera, S. Hirota
    “Dioxygen Binding to a Simple Myoglobin Model in Aqueous Solution”
    Angewandte Chemie International Edition, 44, 435–438 (2005). (DOI: 10.1002/anie.200461609)

  1. (63) K. Kano, H. Kitagishi, S. Tamura, A. Yamada
    “Anion Binding to a Ferric Porphyrin Complexed with Per-
    O-methylated β-Cyclodextrin in Aqueous Solution”
    Journal of the American Chemical Society, 126, 15202–15210 (2004).
    (DOI: 10.1021/ja045472i)

  1. (64) K. Kano, Y. Horiki, T. Mabuchi, H. Kitagishi
    “Heptakis(2,3-di-O-carboxymethyl)-β-cyclodextrin as a pH-sensitive Host”
    Chemsitry Letters, 33, 1086–1087 (2004).
    (DOI: 10.1246/cl.2004.1086)

  1. (65) H. Kitagishi, A. Satake, Y. Kobuke
    “Dynamic Structural Change of a Twisted Hexaporphyrin Complex by Highly Specific Molecular Recognition”
    Inorganic Chemistry, 43, 3394–3405 (2004).
    (DOI: 10.1021/ic049645g)